Vitamin D Enhances Immune Effector Pathways of NK Cells Thus Providing a Mechanistic Explanation for the Increased Effectiveness of Therapeutic Monoclonal Antibodies

Patients with diffuse large cell lymphoma who have an adequate vitamin D supply derive significantly more benefit from immuno-chemotherapy rituximab than patients deficiency; this is especially true for female patients. We already been able to show that increases the antibody-dependent cytotoxicity (ADCC) of NK cells in a sex-dependent manner, but it unclear how makes efficient. Methods: Healthy individuals deficiency were supplemented sufficient levels. isolated blood samples before and after saturation. For transcriptome analysis, we used Affymetrix Gene-Chip 2.0™. Gene expression analysis as well supervised unsupervised pathway performed. Results: Among others “NK cell-associated pathway” increased substitution. Five IFN-α subtypes (2, 4, 6, 7 10) IFN-κ highly expressed are mainly responsible these pathways. In contrast, “interferon-gamma response”, other sets cytokine production chemotaxis showed reduction. Toll-like receptor genes (TLR-8, TLR-7, TLR-2) downregulated and, therefore, decline The same could be shown “ubiquitin-ligase” pathway. Conclusions: Increased several may explain ADCC D-replenished otherwise healthy subjects. Other regulators interferon compensatory upregulated compensation, such receptors those ubiquitin ligase, normalize